e13 5 embryos Search Results


90
Charles River Laboratories embryonic day e13.5 embryos
Embryonic Day E13.5 Embryos, supplied by Charles River Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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embryonic day e13.5 embryos - by Bioz Stars, 2026-03
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90
Harlan Sprague Dawley e13.5 embryos
E13.5 Embryos, supplied by Harlan Sprague Dawley, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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e13.5 embryos - by Bioz Stars, 2026-03
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Harlan Laboratories primary mouse embryo fibroblast (mefs) at e13.5 stage
Primary Mouse Embryo Fibroblast (Mefs) At E13.5 Stage, supplied by Harlan Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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primary mouse embryo fibroblast (mefs) at e13.5 stage - by Bioz Stars, 2026-03
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90
Active Motif a pool of atrial tissues from 57 e13.5 c57bl/6j mouse embryos
A Pool Of Atrial Tissues From 57 E13.5 C57bl/6j Mouse Embryos, supplied by Active Motif, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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90
Janvier Labs microglial cultures derived from e13.5 mouse embryos
<t>Microglial</t> cells were incubated with three different doses of JN403 (100 nM, 1 μM, and 10 μM) for 24 hours, followed by an assessment of cell viability using the MTT-assay. (A) JN403 treatment alone did not elicit cellular toxicity in microglial cells at concentrations up to 1 μM. non-significant (n.s). (B) Microglial cells were pre-treated with 100 nM or 1 μM of JN403 for 24 hours and additionally coincubated for another 24 hours with 1μM αSyn fragment 61-140 on the next day. αSyn induced cytotoxicity in microglial cells. Neither 100 nM nor 1 μM of JN403 treatment changed the cell viability. Data are presented as the mean ± standard error of the mean. Cell viability was measured for multiple independent experiments. **** p < 0.0001, significantly different from the control group.
Microglial Cultures Derived From E13.5 Mouse Embryos, supplied by Janvier Labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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microglial cultures derived from e13.5 mouse embryos - by Bioz Stars, 2026-03
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90
Envigo animals e13.5 embryos from timed pregnant hsd:nsa (cf-1) mice
<t>Microglial</t> cells were incubated with three different doses of JN403 (100 nM, 1 μM, and 10 μM) for 24 hours, followed by an assessment of cell viability using the MTT-assay. (A) JN403 treatment alone did not elicit cellular toxicity in microglial cells at concentrations up to 1 μM. non-significant (n.s). (B) Microglial cells were pre-treated with 100 nM or 1 μM of JN403 for 24 hours and additionally coincubated for another 24 hours with 1μM αSyn fragment 61-140 on the next day. αSyn induced cytotoxicity in microglial cells. Neither 100 nM nor 1 μM of JN403 treatment changed the cell viability. Data are presented as the mean ± standard error of the mean. Cell viability was measured for multiple independent experiments. **** p < 0.0001, significantly different from the control group.
Animals E13.5 Embryos From Timed Pregnant Hsd:Nsa (Cf 1) Mice, supplied by Envigo, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/animals e13.5 embryos from timed pregnant hsd:nsa (cf-1) mice/product/Envigo
Average 90 stars, based on 1 article reviews
animals e13.5 embryos from timed pregnant hsd:nsa (cf-1) mice - by Bioz Stars, 2026-03
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Microglial cells were incubated with three different doses of JN403 (100 nM, 1 μM, and 10 μM) for 24 hours, followed by an assessment of cell viability using the MTT-assay. (A) JN403 treatment alone did not elicit cellular toxicity in microglial cells at concentrations up to 1 μM. non-significant (n.s). (B) Microglial cells were pre-treated with 100 nM or 1 μM of JN403 for 24 hours and additionally coincubated for another 24 hours with 1μM αSyn fragment 61-140 on the next day. αSyn induced cytotoxicity in microglial cells. Neither 100 nM nor 1 μM of JN403 treatment changed the cell viability. Data are presented as the mean ± standard error of the mean. Cell viability was measured for multiple independent experiments. **** p < 0.0001, significantly different from the control group.

Journal: bioRxiv

Article Title: JN403, an alpha-7-nicotine-acetylcholine-receptor agonist, reduces alpha-synuclein induced inflammatory parameters of in vitro microglia but fails to attenuate the reduction of TH positive nigral neurons in a focal alpha-synuclein overexpression mouse model of Parkinson’s disease

doi: 10.1101/2020.04.04.996892

Figure Lengend Snippet: Microglial cells were incubated with three different doses of JN403 (100 nM, 1 μM, and 10 μM) for 24 hours, followed by an assessment of cell viability using the MTT-assay. (A) JN403 treatment alone did not elicit cellular toxicity in microglial cells at concentrations up to 1 μM. non-significant (n.s). (B) Microglial cells were pre-treated with 100 nM or 1 μM of JN403 for 24 hours and additionally coincubated for another 24 hours with 1μM αSyn fragment 61-140 on the next day. αSyn induced cytotoxicity in microglial cells. Neither 100 nM nor 1 μM of JN403 treatment changed the cell viability. Data are presented as the mean ± standard error of the mean. Cell viability was measured for multiple independent experiments. **** p < 0.0001, significantly different from the control group.

Article Snippet: Briefly, microglial cultures derived from E13.5 mouse embryos (Janvier Breeding Center, France) were obtained by mild trypsinization as described previously.

Techniques: Incubation, MTT Assay, Control

Inflammatory parameters (A) Nitric Oxide (NO) and (B) TNF-α were measured in JN403 pre-treated microglial cells for a baseline. (A) NO, and (B) TNF-α release increased with 1μM αSyn incubation, but both 100 nM and 1 μM of JN403 co-treatment significantly reduced NO and TNF-α release, * p < 0.05, ** p < 0.01.

Journal: bioRxiv

Article Title: JN403, an alpha-7-nicotine-acetylcholine-receptor agonist, reduces alpha-synuclein induced inflammatory parameters of in vitro microglia but fails to attenuate the reduction of TH positive nigral neurons in a focal alpha-synuclein overexpression mouse model of Parkinson’s disease

doi: 10.1101/2020.04.04.996892

Figure Lengend Snippet: Inflammatory parameters (A) Nitric Oxide (NO) and (B) TNF-α were measured in JN403 pre-treated microglial cells for a baseline. (A) NO, and (B) TNF-α release increased with 1μM αSyn incubation, but both 100 nM and 1 μM of JN403 co-treatment significantly reduced NO and TNF-α release, * p < 0.05, ** p < 0.01.

Article Snippet: Briefly, microglial cultures derived from E13.5 mouse embryos (Janvier Breeding Center, France) were obtained by mild trypsinization as described previously.

Techniques: Incubation

Iba1+ microglial cells were detected and the density analysis showed no differences between the rAAV groups and treatment groups. (A) Representative photomicrographs showing Iba1+ signals per group, TH (green) and Iba1 (magenta). No activated forms of microglia were found. Scale bar 200 μm. (B) The ratio of Iba1+ density of the injected side compared to the non-injected side showed no significant differences between the rAAVs and treatment, n=4, non-significant (n.s).

Journal: bioRxiv

Article Title: JN403, an alpha-7-nicotine-acetylcholine-receptor agonist, reduces alpha-synuclein induced inflammatory parameters of in vitro microglia but fails to attenuate the reduction of TH positive nigral neurons in a focal alpha-synuclein overexpression mouse model of Parkinson’s disease

doi: 10.1101/2020.04.04.996892

Figure Lengend Snippet: Iba1+ microglial cells were detected and the density analysis showed no differences between the rAAV groups and treatment groups. (A) Representative photomicrographs showing Iba1+ signals per group, TH (green) and Iba1 (magenta). No activated forms of microglia were found. Scale bar 200 μm. (B) The ratio of Iba1+ density of the injected side compared to the non-injected side showed no significant differences between the rAAVs and treatment, n=4, non-significant (n.s).

Article Snippet: Briefly, microglial cultures derived from E13.5 mouse embryos (Janvier Breeding Center, France) were obtained by mild trypsinization as described previously.

Techniques: Injection